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@#Objective To assess the feasibility and safety of ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy in selected patients with primary palmar hyperhidrosis. Methods From March 1, 2018 to February 1, 2021, 90 patients with primary palmar hyperhidrosis who underwent ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy at the Thoracic Surgery Department of the University of Hong Kong-Shenzhen Hospital. There were 47 males and 43 females, with a median age of 26.0 (22.0, 31.0) years. During the operation, T3 and/or T4 thoracic sympathetic nerve chain was transected using an ultra-micro 5 mm single-port incision near the areola or under the axilla. The surgical data of the patients were retrospectively reviewed and analyzed. Results All patients successfully completed the operation without major bleeding during the operation and no conversion to thoracotomy. There was no death or serious complication during the perioperative period. The operation time was 43.0 (23.0, 60.0) min, and the intraoperative blood loss was 2.0 (1.0, 2.0) mL. In the perioperative period, only one patient needed a tiny chest tube indwelling. The symptoms of hyperhidrosis on the hands all disappeared after the operation. The pain score on the postoperative day was 2.0 (2.0, 2.0) points. The hospital stay after surgery was 1.0 (1.0, 1.0) d. In the first month after the operation, the symptoms of hyperhidrosis on the hands were significantly relieved compared with those before the operation. The surgical incisions healed well, the wounds were concealed, and there was no wound infection or poor healing. The patients' satisfaction with the surgical incisions was 100.0%. After the operation, 14 (15.6%) patients had mild compensatory hyperhidrosis, 5 (5.6%) patients had moderate compensatory hyperhidrosis, and no patient had severe compensatory hyperhidrosis. Overall satisfaction rate was 94.0%. Conclusion The clinical application of ultra-micro 5 mm single-port endoscopic thoracic sympathicotomy in selected patients with primary palmar hyperhidrosis is safe and feasible. The surgical wound is extremely small and hidden, the operation time is short, the pain is very slight, and the clinical outcome is good. It can fully meet the patients' pursuit of beauty.
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OBJECTIVE:To establish HPLC fingerprint for the leaves of Camptotheca acuminante in Guizhou.METHODS:HPLC method was performed.The determination was performed on Gemini-NX C18 column with mobile phase consisted of acetonitrile-0.2％ phosphoric acid (gradient elution) at the flow rate of 1.0 mL/min.The detection wavelength was set at 370 ran,and the column temperature maintained at 30 2.The sample size was 10 μtL.Using sorbitol as a reference,HPLC fingerprints of 14 batches of the leaves of C.acuminante were determined.The chromatographic fingerprint was analyzed with Similarity Evaluation System for Chromatographic Fingerprint of TCM (2004 A) in terms of common peak indentification,similarity evaluation and cluster analysis.RESULTS:There were 10 common peaks in HPLC fingerprints for 14 batches of the leaves of C.acuminate.And the similarity of 13 batches of the leaves of C.acuminate was greater than 0.90,and that of another one was less than 0.90.The leaves of C.acuminate were classified into 3 groups.CONCLUSIONS:The established fingerprint can provide reference for identification and quality evaluation of the leaves of C.acuminate.
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Objective To develop a new method to measure pleural effusion volume by ultrasound in critically ill patients. Methods Forty-six critically ill patients admitted to emergency ICU were involved.The height of effusion (H),area of effusion at the middle section (S), thickness of effusion at middle-back line (T1) and posterior axillary line (T2) were measured by ultrasound in supine position at the end of expiration. The measured volume of pleural effusion (Vc) was calculated by H×S,and the actual volume of drainage (V) within 2 hours was also recorded. The correlation of actual volume of pleural effusion (V)with effusion height (H) ,thickness (T1, T2), area (S) and the calculated volume (Vc) were analyzed to decide the most accurate index and method. Results There was much better correlation between actual volume of effusion and S, (H & S), Vc, than these between V and T1 ,T2, H in all patients and subgroup, Vc had good correlation with V and very close to V(the average difference was 56 ml) when the actual volume was less than 500 ml,there was no difference[(417 ± 94)ml vs (402±95)ml, t = 1.095, P = 0. 285]. Both Logistic regression analysis and receiver operating characteristic (ROC) curve showed S was the most reliable index to predict the actual volume to exceed 500 ml,400 ml,and 300 ml when compared with H,S,T1 and T2. The corresponding threshold was 30.3 cm2 , 28.3 cm2 and 23. 1 cm2 , with the sensitivity and specificity of 0. 77 and 0. 88,0.72 and 1.0,0.95 and 1.0, respectively. Conclusions This new method based on measuring the area of effusion by ultrasound is more efficient and reliable than those traditional ones to measure the volume of pleural effusion. It's clinically valuable and easy to perform, and deserves broad application.
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OBJECTIVE:To establish the quality standard of Patch for hyperosteogeny.METHODS:Mastic and Myrrh in Patch for hyperosteogeny were qualitatively identified by TLC,and the content of paeoniflorin in preparation were determined by HPLC.RESULTS:The identification of TLC has a good resolution and specificity.The linear range of paeoniflorin were 0.204～1.428 ?g(r=0.999 2) with an average recovery of 99.41%(RSD=0.64%,n=6).CONCLUSION:Established standard can be used for the quality control of Patch for hyperosteogeny.
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Objective To study the effect of growth hormone (GH) on circulating neutrophil activation in septic peritonitis rats induced by lipopolysaceharide.Methods Male Wistar rats were divided into six groups (n=7) in randomized manner:normal control,septic peritonitis,septic peritonitis plus GH (0.5 mg/kg),septic peritonitis plus GH (1.0mg/kg),septic peritonitis plus GH (2.0 mg/kg),GH alone (2.0 mg/kg).Septic peritonitis rats were induced by lipopolysaccharide.CD11b expression and an oxidative burst of neutrophils were detected by flow cytornetric analysis. Results Growth hormone significantly increased sepsis-induced expression of CD11b and oxidative burst of circulating neutrophils (P＜0.01).Control animals treated with GH alone had CD11b expression and oxidative burst similar to that animals,treated with saline.Conclusion Treatment of GH may be harmful,instead of beneficial,on sepsis-induced organ injury.
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Objective Establishing a kind of analysis method about HPLC character spectra of native medicinal materials, Lonicera fulvotomentosa, in Guizhou province for supplying experiment basis to guide processing and quality control of Lonicera fulvotomentosa. Methods Making the Lonicera fulvotomentosa sample liquors with 50% carbinol solvent and vibrating 30 minutes by ultrasonic. Adopting high performance liquid chromatography RP-HPLC method, Luna C18 (2) (5 ?m, 250 mm ? 4.6 mm) chromatographic column, the volume flow 1.0 mL/min, the column temperature 30 ℃, the detection wavelength 238 nm, the mobile phase 0.2% phosphoric acid acetonitrile (A)-0.2% phosphoric acid solution (B), the gradient elution 0～15 min, A∶B=10%∶90%, 15～60 min, A∶B=10%∶90%→40%∶60%. Results Determined 9 common peak in character spectra of the Lonicera fulvotomentosa by detecting 10 group Lonicera fulvotomentosa medicine materials with referring to chlorogenic acid, the precision and stability and reiteration test RSD value all less than 3.0%. The similitude degree between the samples and the character spectras was more than 0.99 by evaluating ten batch of the samples with the similarity assessing soft, proving that the quality of the ten batch of the Lonicera fulvotomentosa medicine materials was stable and homogeneous. Conclusion The character spectra of the Lonicera fulvotomentosa was established to supply experiment basis for effectively controlling process, quality and standard of Lonicera fulvotomentosa.
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N-acetyltransferase are phase II code proteins with overlapping substrate specifici-DMEs, and two highly similar human NAT gene ty. NAT2 is a polymorphic acetyltransferase gene (designated NAT1 and NAT2) are shown to en- locus and "slow acetylation" in humans is due tonutations in the single coding exon of the NAT2 gene. It was also demonstrated that there exist discrete NAT1 structural variants, and differences in tissue levels of NAT1 among humans are related to specific sequence differences in the NAT1 structural gene. Biochemical studies have shown that NAT1 and NAT2 play an important role in the metabolism of some carcinogens, epidemiological studies have revealed an association between ploy-morphisms of NATs and increased cancer risk.Metabolic phenotypes/genotypes can significantly influence DAN adduct formation and could ultimately lead to alterations in cancer risk. If unequivocal biomarkers of genetic susceptibility to cancer can be developed successfully, then identification of individuals at increased risk would be very helpful in the fields of public health and preventive medicine.